Fig. 2

a. (left) Plot of top Gene Ontology (GO) gene set by GSAA for ZMEL1-INV vs. -PRO RNA-seq. (right) Dual waterfall plot of top/bottom 250 gene sets from GO analysis (for full plot see Figure S2a). Adhesion GO gene sets are indicated with an asterisk and colored according to false discovery rate (FDR) b. Heatmap of genes in adhesion GO gene sets (FDR < 0.05, n=3) that are differentially expressed between ZMEL1-PRO and -INV (log₂ fold change cutoff ± 1.5, p_adj < 0.05). Human ortholog gene names are used for clarity (see Figure S2b-c for absolute expression and zebrafish gene names). c. (top) Schematic of assay and (bottom) quantification of cluster formation in low-bind plates after 2 days (N=6 independent experiments, p<0.001 by two-sided t-test, plot shows mean ± SD). d. Representative images of clusters formed after 3 days. e. Human melanoma cell lines ranked by cluster forming ability (left to right: low to high) with PRO/INV gene expression scores (Hoek et al., 2006) (Spearman correlation and Bonferroni-corrected p-values shown, scale bar 100μm). f. Co-clusters of 1:1 mixture of ZMEL1-PRO and -INV (inset scale 50 μm). g. (left) 3D opacity rendering and (right) central slice (slice 54 of 115) of confocal imaging of co-cluster of ZMEL1-PRO and ZMEL1-INV. h. Co-clusters of 1:1 mixture of ZMEL1-INV and ZMEL1-INV with either control (sg_scr) or cdh1 (sg_cdh1) sgRNA (inset scale 50 μm). f-h are composite images. See also Figure S2, Tables S1, S5,S6, and Videos S2,S3.