|
Fig. 4
Figure 4. Assign beads and bonds. (A) BOC from Fig. 1 D. Individual chains are colored differently. Solid and dashed arrows (paths 1 and 2) denote counterclockwise and clockwise search directions along the bead boundary from the arbitrarily chosen seed bead (circled) to construct the outer chain. (B and C) Zoomed-in views of the rectangles in (A). One grid window represents one pixel. Beads and bonds are added as new edges meet the bond length cutoff b0. Beads are assigned at pixel corners. (C) For boundary forming a closed contour, only one search direction is needed. The last bond added to close the contour is marked in cyan. Note that the sharp bend marked by a star is retained after smoothing (cf. Fig. S2).