ZFIN Image: Sander et al., 2021, Fig. 2

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Fig. 2

Mitochondrial Na⁺/Ca²⁺ exchanger-mediated mitochondrial Ca²⁺ extrusion. Free Ca²⁺ in the supernatant of permeabilized HeLa cells was recorded using Calcium Green-5N before and after injection of 100-μM Ca²⁺ (first arrowhead) followed by a block of mitochondrial Ca²⁺ uniporter using Ru360 (second arrowhead). Addition of 60-mM Na⁺ (third arrowhead) induced mitochondrial Na⁺/Ca²⁺ exchanger-mediated Ca²⁺ extrusion (control, black trace) which could be blocked by the mitochondrial Na⁺/Ca²⁺ exchanger blocker CGP-37157 (control, red trace). Neither ezetimibe, nor disulfiram or honokiol affected NCLX activity (n = 4)

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