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Fig. 1 Fig. 1. Example for a schematic layout of optimization strategies. 1?2 × 105 ZF4 cells were seeded in 24-well plates. Transfection experiments were proceeded at a final confluence of 50%?70%. Numbers in each well refers to trial-1?3 of each transfection reagent. All transfection methods of an optimization experiment were proceeded at the same time. Optimization was repeated three times for individual experiments.
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