Fig. 11

Fig. 11 Fig. 11. Localization of 5HT+ soma in the episphere. A–D) ASW (A) and mock (B–D) treatment animals had a symmetric bimodal distribution of 5HT+ cells, indicative of a left and right brain lobe. E–I) 1q (E), 2q (F–H), and 3q (I) pulse BMP treatment animals showed a uniform distribution of 5HT+ cells, indicative of a radialized brain. J–L) 4q pulse animals showed a bimodal ventral distribution of 5HT+ cells, indicative of a ventralization of the brain. A,B, E,F, I,J) Percentage of animals with 5HT+ cells in each region of the episphere. C,G,K) Immunolabeling of 5HT in 3 treatments representative of the 3 brain states. D,H,I) Merged images showing immunolabeling for 5HT (magenta) and acetylated tubulin (white) and staining for nuclei (Hoechst, cyan). White arrows: Mean vectors(s) of 5HT localization model, scale bars: 50 ​μm.