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Fig. 3
(A) Lateral view schematic of a mouse E10.5 embryo indicating the locations of head and trunk xenotransplantation.
(B–E′) 5 μm optical slices of whole-mount immunolabeling for anti-neurofilament (NF; white) of representative SK-N-AS NB (B–C′) or A375 melanoma (D–E′) cells at 14 hpi in E10.5 mouse embryos. Head injections targeted a region populated by cranial neural crest cells, analogous to that targeted in zebrafish. 71% of assayed embryos (n = 12/17 embryos) with SK-N-AS injections into the head harbored NF-positive cells, whereas 0% of assayed embryos (n = 0/11 embryos) with SK-N-AS injections into the trunk harbored NF-positive cells. No embryos with A375 melanoma injections into the head (n = 0/16 embryos) or trunk (n = 0/14 embryos) harbored NF-positive cells. Scale bar, 10 μm.
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Reprinted from Developmental Cell, 56(19), Treffy, R.W., Rajan, S.G., Jiang, X., Nacke, L.M., Malkana, U.A., Naiche, L.A., Bergey, D.E., Santana, D., Rajagopalan, V., Kitajewski, J.K., O'Bryan, J.P., Saxena, A., Neuroblastoma differentiation in vivo excludes cranial tumors, 2752-2764.e6, Copyright (2021) with permission from Elsevier. Full text @ Dev. Cell