ZFIN Image: Watson et al., 2022, Fig 3

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Figure Caption

Fig 3

wnt16 is required for notochord and myotome morphogenesis.

(A) Brightfield images of wnt16^-/- mutants and clutchmate controls. Insets show closeup of notochord. (B) wnt16^-/- mutants exhibit significantly reduced standard length at 3 dpf. (C-F) Myotome morphology (C-E) and notochord height (F) are altered in wnt16^-/- mutants. Insets provide schematic of measurement. (G) Lateral views of phalloidin-stained 3dpf animals obtained using confocal microscopy. (H-I) Quantitative image analysis reveals that myofibril angle (H) but not myofibril length (I) or nuclei per myotome area (J) are altered in wnt16^-/- mutants. P-values were determined using either two-way ANOVA with Fisher’s LSD post hoc test (B) or an unpaired t-test (C-F, H-J). *p<0.05, **p<0.01, ****p<0.0001, ns: not significant.

Acknowledgments

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