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Fig 3 Clutches from cd9b mutant pairs, produced by IVF, have a decreased clutch size and lower percentage of eggs fertilised per clutch.
The number of eggs is significantly decreased in clutches from cd9b mutant pairs (A). Of the eggs laid, a significantly lower percentage were fertilised by cd9b mutant males (B). Dead eggs were immediately discarded during the IVF protocol and therefore (A) and (B) represent only fertilised and unfertilised eggs. n = minimum 3 pairs per genotype. ANOVA with Holm-Sidak multiple comparisons test was carried out on the original data from (A) and on arcsine transformed data in (B). The Holm-Sidak post-hoc test was chosen as it has more power than Dunnett’s multiple comparisons test. Significance of difference from WT control: ** p<0.01; * p<0.05; ns = not significant. Data from a single experiment.