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FIGURE 7 Maternal and zygotic expression of gne WT and gne KO alleles. (A) gne maternal inheritance. gne+/- in-cross results in 3 genotypic groups. gne KO embryos are predicted to receive both gne+ and gne− maternal transcripts (blue arrow), but the zygotic transcription will only transcribe gne− alleles (red arrow). (B) Maternal expression of gne WT and gne mutant allele at 1 and 3 hpf embryos. The relative quantification (RQ) values of gne WT (blue bars) and gne mutant alleles (red bars) are shown by genotype and age. The WT allele was highly expressed in the WT samples and was lower in gne+/- offspring at 1 and 3 hpf. No amplification was obtained for the gne KO allele. cDNA sample of gne HT at 3 dpf was used as a calibrator for all reactions since it expresses both the WT and the KO alleles. (C) The relative expression of gne WT and gne KO alleles at 3, 5, and 7 dpf. The WT allele was highly expressed in the WT samples but was not found in gne KO at all-time points, and the mutated allele showed the opposite behavior.