ZFIN Image: He et al., 2022, Figure 3

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Figure 3

Effect of the DOTAP/MC3 ratios in dmLNP formulations on cell association and transfection in activated HUVEC. (A) Endothelial association of dmLNP formulations with increasing DOTAP/MC3 ratios (5D/45M, 10D/40M and 25D/25M). TNF-α activated HUVEC were incubated with LNP for 3 h, and EC-associated-DiI fluorescence was determined by flow cytometry. Data are normalized MFI ± SD of three independent experiments. (B) SiRNA-based gene silencing in activated EC mediated by the LNP formulations with different DOTAP/MC3 ratios, at the siRNA concentration of 15 nM or 60 nM. Data are from 3 independent experiments and shown as mRNA fold change ± SD.

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