ZFIN Image: Farhan et al., 2022, Figure 4

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Figure Caption

Figure 4

Validation of the performance of OpenBloodFlow GUI package for blood flow velocity measurement through comparison with ImageJ method. Zebrafish embryos aged (A) 2 dpf, (B) 3 dpf, (C) 4 dpf, or (D) 5 dpf were subjected to ImageJ and OpenCV methods to measure the average blood flow velocity. The data were shown as mean with standard deviation (circle dots show the data analyzed by OpenCV, while square dots show the data analyzed by ImageJ), and the significant difference was calculated by paired t-test. (ns = no significance, n = 18–26).

Acknowledgments

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