Figure 12

Oligo-fucoidan reduces the elevated senescence-associated ?-galactosidase (SA-?-gal) expression by WNK1?OSR1 axis inhibitors in tert transgenic fish. We use Tg(fabp10a:tert; myl7:GFP) transgenic fish to further verify whether drugs can prevent cellular senescence. These fish were fed with a normal diet from 5 dpf to 10 dpf old and treated with several drugs: Regorafenib (0.5 ?M), oligo-fucoidan (0.3 mg/mL), WNK463 (0.625 ?M) with or without oligo-fucoidan, Rafoxanide(0.3 ?M) with or without oligo-fucoidan, and were then fixed and stained on 11 dpf. (a) The SA-?-gal expression in various combinations of drug-treated tert transgenic fish after various drug treatments. WT: AB(WT) fish as control, tert: Tg(fabp10a:tert; myl7:GFP) fish; OF: oligo-fucoidan-treated tert transgenic fish; WNK463: WNK1 inhibitor WNK463-treated tert transgenic fish; WNK463+OF: WNK463 plus oligo-fucoidan-treated tert transgenic fish; Rafox: OSR1 inhibitor Rafoxanide-treated tert transgenic fish; Rafox+OF: Rafoxanide plus oligo-fucoidan-treated tert transgenic fish. The scale bar is 100 µm in length. The orange circle indicates the location of the liver. (b) Quantification of SA-?-gal staining. For each experiment, we performed three replicates. Ten larvae were included in each experiment. One-way ANOVA analysis followed by multiple analysis of variance was used to identify the statistical significance between groups. *: p ? 0.05; **: p ? 0.01; ns: no-significance.