ZFIN Image: Hou et al., 2022, Figure 4

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Figure 4

The expression of WNK1 and OSR1 is upregulated in hepatoma cells during tumor-induced angiogenesis promoting hepatoma cell proliferation. The expression of WNK1 and its downstream effectors in hepatoma cells during tumor induced angiogenesis. (a) WNK1, (b) OSR1, (c) STK39, (d) PPP2R1B in zebrafish embryos carrying hepatoma cells from 0 dpi to 3 dpi during tumor-induced angiogenesis. We performed three to four replicates, and for each qPCR, there were triplicates for each sample. Thirty embryos were included in each experiment. One-way ANOVA analysis followed by multiple analysis was used to identify the statistical significance between groups. *: p ? 0.05; **: p ? 0.01; ***: p ? 0.001; ****: p ? 0.0001; ns: no-significance.

Acknowledgments

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