Embryonic nicotine exposure effects viscerocranial cartilages length. (A) Viscerocranial cartilages measurements. (B) Meckel’s length, (C) Ceratohyal length, (D) Palatoquadrate length, and (E) Hyosimpletic length. The size of all viscerocranial cartilages decreased with embryonic exposure to nicotine in a dose-dependent manner. Sample sizes are: DMSO n = 31; 12.5 µM nicotine n = 30; 25 µM nicotine n = 30; and 50 µM nicotine n = 34; m, meckel’s cartilage; ch, ceratohyal; pq, palatoquadrate; hs, hyosimpletic. * = p < 0.05; ** = p < 0.01; *** = p < 0.001; **** = p < 0.0001. All images were captured at 10× magnification. Scale bar 50 µm.
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