ZFIN Image: Müller et al., 2022, Fig. 2

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Figure Caption

Fig. 2 Characterization of ELC interacting kinases.

A NEK9 and CamK2G RNA expression in human total RNA tissue panel. Ribosomal protein large subunit P0 (RPLP0) was used as housekeeping gene. B NEK9 (left panel) and CamK2G (right panel) protein expression in piscine and human heart tissue to detect cross-species applicability. C mRNA expression profile of NEK9 and CamK2G in different human tissues from 4 weeks post-conception to adulthood⁶⁸. D, E Relationship of NEK9-ELC binding efficiency on intracellular Ca²⁺-concentration. NEK9-ELC interaction was analyzed after overexpression of myc-tagged ELC protein in human cells followed by specific immunoprecipitation (IP) and immunoblot (IB) analysis (D). Ca²⁺-influx was obtained by ionophore stimulation. Quantification of NEK9-ELC interaction dependent on Ca²⁺-influx illustrated as the averaged intensity of precipitated NEK9 protein normalized to actin protein expression of input protein lysate (E). Data are mean ± SD (n = 3). **P < 0.01 by the analysis of ordinary one-way ANOVA followed by Bonferroni’s multiple comparisons test.

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