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Fig. 1
Schematic procedure for the generation of the transgenic zebrafish model for pancreatic beta cell regeneration. The recombinant Tol2 plasmid and transposase mRNA were co-injected into the one-cell stage of zebrafish zygotes. The injected embryos were evaluated at 2 dpf using fluorescence stereo-microscope, and EGFP positive embryos were selected. EGFP positive embryos raised as F0 generation of Tg(ins:egfp-nsfB) zebrafish. Then each one of the F0 generation adult zebrafish was crossed with a wild-type (TU stain) zebrafish to produce F1 generation.
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Full text @ Galen med. j.