Figure 6

Figure 6

Co-localization of Bucky ball and CVH during stages EK I to EK III. During the first two cleavage stages (EK I and EK III), Bucky ball strictly co-localized to CVH within the cleavage furrows and in condensed granules in the cleavage center A: (A1) stitched fluorescent overlay images of the central region of an EGK II embryo with the indicated region of high resolution images shown in (A2–A4) (A2) Bucky ball, (A3) CVH, (A4) overlay. (A2—A4) are included in a preprint⁵¹. During the next stage, EK III, both germ plasm proteins translocate into the cytoplasm of the first forming central cells B: (B1) transmission image of the total embryonic disc at stage EGK III with indicated central region for high resolution fluorescent imaging: (B2) Bucky ball, (B3) CVH, (B4) overlay. Asterisk indicate the diffusion of Bucky ball and CVH proteins into the cytoplasm. Scales 20 µm, except (B1) = 500 µm.