ZFIN Image: Xing et al., 2022, Figure 6

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Figure Caption

Figure 6

Myt1 is necessary for the development of motor neurons.A–C, transverse sections of spinal cord showing myt1a RNA (blue) in Tg(mnx1:EGFP) (pink) embryos. Dorsal top, rostral left. The scale bar represents 25 μm. D–I, confocal imaging analysis of primary motor neurons in control, KO myt1a/b, and mRNA rescue groups at 48 hpf (D–F) and 72 hpf (G–I) Tg(mnx1:GFP). Arrowhead denotes round-shaped and undifferentiated neuronal cells. The scale bar represents 50 μm. Arrows denote missing or shortened MiP or CaP axons. Dashline in (H) shows CaP axons at 72 hpf. Enlarged images of (D–I) are available at Fig. S7. J, schematic diagram of three primary motor axon projections (CaP, MiP, and RoP) in zebrafish. K, the length of CaP axons of zebrafish in the control group, in the KO myt1a/b group, and in the mRNA rescue group at 48 hpf (n = 8 in each group, ∗∗∗∗p < 0.0001, one-way ANOVA followed by Turkey’s test) and 72 hpf (n = 8 in each group, ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, one-way ANOVA followed by Turkey’s test). L, the percentages of defective CaP axons in the control group, the KO myt1a/b group, and in the mRNA rescue group at 48 hpf (n = 8 in each group, ∗∗∗∗p < 0.0001, one-way ANOVA followed by Turkey’s test) and 72 hpf (n = 8 in each group, ∗∗∗∗p < 0.0001, one-way ANOVA followed by Turkey’s test). M, the percentages of defective MiP axons in the control group, the KO myt1a/b group, and mRNA rescue group at 48 hpf (n = 8 in each group, ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, one-way ANOVA followed by Turkey’s test) and 72 hpf (n = 8 in each group, ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, one-way ANOVA followed by Turkey’s test). N and O, distance and velocity analysis in the control group, the KO myt1a/b group, and mRNA rescue group in 5 dpf larvae (n = 9, respectively, ∗∗p < 0.01, ∗p < 0.05, one-way ANOVA followed by Turkey’s test) by the video tracking software EthoVision XT. P, turn angle in the control group, the KO myt1a/b group, and mRNA rescue group in 5 dpf larvae (n = 9, ∗∗∗∗p < 0.0001, one-way ANOVA followed by Turkey’s test). All data were shown as the mean ± SD. ∗, ∗∗, ∗∗∗, and ∗∗∗∗ denote p < 0.05, p < 0.01, p < 0.001, p < 0.0001, respectively. n.s., not significant. hpf, hour post fertilization.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biol. Chem.