Fig. 4

Fig. 4

Fig. 4. Immunostaining for Gfap on retinal cryosections of Tg(CMV:GFP-Lc3) autophagy reporter fish, three days after ONC. Representative images disclosed a clear co-localization of the Lc3 (green) and Gfap (red) signal in the IPL, as well as in the NFL, where the end feet of the Müller glia are positioned. Scale bar = 25 µm. Representative image of n = 4. Dpi, days post-injury; GFP, green fluorescent protein; INL, inner nuclear layer; IPL, inner plexiform layer; Gfap, glial fibrillary acidic protein; Lc3, microtubule-associated protein 1A/1B-light chain 3; NFL, nerve fiber layer; RGCL, retinal ganglion cell layer. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)