Fig. 2

Fig. 2

Fig. 2. Visualization of the autophagic response in the NFL and RGCL at different time points after ONC using retinal whole mounts of Tg(CMV:GFP-Lc3) autophagy reporter fish. (A) In the NFL of the fish retina, GFP-Lc3 fluorescence intensity was significantly increased from 1 dpi and reached its maximum value at 3 dpi. Hereafter, the Lc3 reporter signal decreased and baseline levels were reached two weeks after injury. (B) A similar trend was observed for the Lc3 signal in the RGCL. Scale bar = 25 µm. Representative images of n = 4. Dpi, days post-injury; GFP, green fluorescent protein; Lc3, microtubule-associated protein 1A/1B-light chain 3; NFL, nerve fiber layer; RGCL, retinal ganglion cell layer.