Fig. 1

Fig. 1

Pronephron defects in osr1 mutants are partially suppressed by hand2 loss of function. (A) osr1^el593 is a TALEN-generated 7 bp deletion allele. Schematics show gene structure with location of deletion, and predicted wild-type and Osr1^el593 proteins (ZF, zinc finger). (B,C) Lateral views, anterior to the left, of wild-type (B) and osr1 mutant (C) embryos at 4 days post-fertilization (dpf). osr1 mutants display severe pericardial and body wall edema (arrows). (D-T) Dorsal views, anterior to the left, of a pronephron schematic (D), and wild-type (E,I,M,Q), osr1 mutant (F,J,N,R), hand2 mutant (G,K,O,S) and hand2; osr1 double mutant (H,L,P,T) embryos at 26 h post-fertilization (hpf). In situ hybridization shows expression of wt1b (E-H) in glomerular precursors, cdh17 (I-L) throughout the tubules, slc20a1a (M-P) in the proximal convoluted tubules, and slc12a3 (Q-T) in the distal late segments. Compared with wild type (E,I,M,Q), expression is absent (F), thin and shortened anteriorly (J), reduced (N), and thin (R) in osr1 mutants; expanded in hand2 mutants (G,K,O,S); and relatively similar to wild type in hand2; osr1 double mutants (H,L,P,T). Scale bars: 200 µm (B,C); 100 µm (I-T); 25 µm (E-H).