IMAGE

FIGURE 6

ID

ZDB-IMAGE-220924-6

Genes

EGFP
GFP

Publication

Wareham et al., 2022 - Adamts10 controls transforming growth factor β family signaling that contributes to retinal ganglion cell development

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Figures for Wareham et al., 2022

Image

Figure Caption

FIGURE 6

Inhibition of TGFβ signaling with SB431542 reduces pou4f1 enhancer-driven GFP expression in the retina. Representative images of untreated, DMSO-treated and SB431542-treated Tg(12xSBE:EGFP) embryos at 48 hpf (A) showing GFP fluorescence reporting of pSmad3-mediated signaling in the retina (white arrows), brain (magenta arrows) and lens (blue arrows); scale = 250 µm. Representative images of untreated, DMSO-treated and SB431542-treated Tg(pou4f1-hsp70l:GFP) embryos at 48 hpf (B) showing pou4f1 enhancer-driven GFP fluorescence in the retina (white arrows) and optic nerves (magenta arrows); scale = 250 µm. Normalized integrated fluorescence density (C) of each condition (Tg(12xSBE:EGFP); untreated: n = 7, DMSO-treated: n = 7, SB431542-treated: n = 12: ****p < 0.0001, ***p = 0.0004, ns = non-significant; Tg(pou4f1-hsp70l:GFP); untreated: n = 11, DMSO-treated: n = 5 and SB431542-treated: n = 8, ****p < 0.0001, ***p = 0.0004, ns = non-significant; one-way ANOVA Tukey’s multiple comparisons test.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Mol Biosci