FIGURE 3

FIGURE 3

Adamts10 MO-induced reduction in pou4f1 enhancer-driven GFP expression is decreased with normal human ADAMTS10 mRNA but not ADAMTS10^G661R mutant mRNA. Representative fluorescent images of a Tg(pou4f1-hsp70l:GFP) embryo uninjected (A) injected with adamts10 MO (B), co-injected with ADAMTS10 MO and normal human mRNA (C) and co-injected with adamts10 MO and ADAMTS10^G661R mRNA (D), showing pou4f1 enhancer-driven fluorescence in the retina (white arrows) and optic nerve (magenta arrows); scale = 250 µm. Corresponding 3D surface plots showing fluorescence intensity for each condition (E–H). Normalized integrated fluorescence density (I) of each condition (uninjected n = 7, adamts10 MO-injected n = 9, normal human mRNA-injected n = 8 and ADAMTS10^G661R mRNA n = 7, *p = 0.03, **p = 0.008,***p = 0.003, ****p < 0.0001; one-way ANOVA Tukey’s multiple comparisons test).