Fig. 3
MRI analyses of beating hearts reveal increased OFT luminal dilation in alk5a?/? adult zebrafish. (A, B) Percentage of OFT outer wall (A) and luminal (B) expansion in WT (n = 10) and alk5a?/? (n = 12) adult zebrafish. Plots show the values for each individual and the mean ± SD; P-values were determined by unpaired t-test (A) or Mann?Whitney test (B). (C?D?) Single frames of MRI cines of WT (C?C?) and alk5a?/? (D?D?) zebrafish in coronal view. Boxed area is shown in C?, D? (minimum expansion OFT) and C?, D? (maximum expansion OFT). Magenta dashed line, OFT lumen; yellow dashed line, OFT outer wall. The colour of each dot refers to the same zebrafish across all graphs and images. The dot adjacent to the number symbol (#) identifies the individual zebrafish mentioned in the text. OFT, outflow tract; V, ventricle. Scale bars: 1.5 mm (C, D), 500 µm (C?, C?, D?, D?).