Figure 3

Figure 3

Craniofacial deformities of adult lrp5 mutant. (A) Adult lrp5^-/- display major deformities of neuro- and viscera- craniums. (B) Schematic representation of morphometrical parameters measured na- nasofacial angle, pd- parasphenoid distance between its edges. (C, D) Nasofacial angle at 3mpf and 6mpf is significantly bigger in lrp5^-/- compared to lrp5^+/+, meanwhile parasphenoid distance is significantly shorter in lrp5^-/- adult fish at 3 and 6mpf (E, F). (G) Parasphenoid bones of lrp5^-/- are severely deformed and display a similar fracture pattern, outlined in yellow. (H) 40% of lrp5^-/- fish displayed malformed parasphenoid at 3mpf and 38.4% at 6mpf within lrp5^-/- group. At 3mpf and 6mpf lrp5^+/+ display no indication of parasphenoid deformities.(Scale bar 500µm, 3mpf n =13-15 fish, 6mpf n =12-13 fish, t-test).