FIGURE 5

FIGURE 5

Tmem184a and Vegfr2 Function Synergistically to Modulate Angiogenesis. (A,A′) Standard control MO injected embryos (n = 35). (B,B′) Vegfr2 MO injected embryos (n = 20). Asterisks indicate ISVs that have failed to complete growth. (C,C′) Embryos injected with the subthreshold concentration of the Tmem184a ATG MO (0.50 mM) (n = 14). (D,D′) Embryos injected with the subthreshold concentration of the Vegfr2 MO (0.25 mM) (n = 8). (E,E′) 48 hpf embryos co-injected with both subthreshold concentrations of the Tmem184a ATG and Vegfr2 MOs (n = 37). Asterisks indicate ISVs that failed to complete growth. (F) This graph illustrates the mean number of intact ISVs ± SEM in 1 mm Vegfr2 and SC MOs. p < 0.01. (G) The graph is the mean number of intact ISVs ± SEM in subthreshold injected Tmem184a ATG and Vegfr2 MOs as compared to both subthreshold MOs. p < 0.01. (H) Embryo survival for all treatments illustrated relative to 24 h survival of SC embryos.