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Fig. 3

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Publication: Stock et al., 2022 - A self-generated Toddler gradient guides mesodermal cell migration
All Figures: Figures for Stock et al., 2022

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Fig. 3

Fig. 3. Rescue of the toddler^−/− phenotype is independent of the Toddler sources’ location.

Ectopic expression of Toddler in toddler^−/− embryos according to the schematic representation of embryos at 75% epiboly (top). Animal pole source: transplantation of Toddler-overexpressing cells to the animal pole of sphere stage toddler^−/− embryos; ubiquitous expression: injection of 2 pg of toddler mRNA (rescuing concentration) into one-cell stage toddler^−/− embryos; marginal source: injection of 10 pg of toddler mRNA into the YSL of 1k-cell stage toddler^−/− embryos. Toddler (red); Dextran control (gray). Mesoderm spread was assessed using in situ hybridization for aplnrb (middle; lateral view, dorsal on the right; white line indicates measurement of ventral mesoderm spread) and quantified relative to the average spread in wild-type embryos (bottom). Data are means ± SD. Significance was determined using one-way ANOVA with multiple comparison; ****P < 0.0001; **P < 0.01. Scale bars, 20 μm.

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