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Fig. 3
Figure 3. Cell fusion events are biased along, but not restricted to, the AP axis and do not show a local bias in position (A) Angle of fusion events relative to tissue AP axis. Left: cartoon of angle definition, with the data distribution shown as a rose plot and the total number of events represented by increasing radius. Red bracket highlights the fraction of cells with fusion angle >30°. (B) As (A), but for the angle of fusion events relative to tissue ML axis. Red bracket highlights the fraction of cells with fusion angle away from ML axis by more than 30°. (A and B) 84 fusion events from 2 embryos. (C) Example of fusion events along different axes. Left: two fast myocytes fusing end-to-end. Right: two fast myocytes fusing side-by-side. Time t = 0 represents fusion time. (D) Left: location of fusion initiation within each fusing cell. Right: orientation of fusing pairs relative to each other. (E) Distribution of end-to-end and side-to-side fusion events. Position 0 μm in each axis corresponds to the segment center, determined by the average cell position. (D and E) 90 cells, 3 myotome segments from 1 embryo.
Reprinted from Developmental Cell, 57(17), Mendieta-Serrano, M.A., Dhar, S., Ng, B.H., Narayanan, R., Lee, J.J.Y., Ong, H.T., Toh, P.J.Y., Röllin, A., Roy, S., Saunders, T.E., Slow muscles guide fast myocyte fusion to ensure robust myotome formation despite the high spatiotemporal stochasticity of fusion events, 2095-2110.e5, Copyright (2022) with permission from Elsevier. Full text @ Dev. Cell