|
Fig. 3
a Confocal images of Dr-Vsp immunostaining showing the subcellular distribution of Dr-Vsp in larval LREs. Dr-Vsp is primarily localized in the cytoplasm underneath the apical surface but barely detectable at the microvilli and plasma membrane of LREs. Green, Dr-Vsp. Red, F-actin, Blue, DAPI. *Vacuole. Scale bar = 10 µm. b Confocal image of larval LREs at higher magnification and their corresponding schematic illustrations, focusing on the intracellular distribution of Dr-Vsp. Green, Dr-Vsp. Red, F-actin. V vacuole, MV microvilli, L lumen. Scale bar = 5 µm. c Fluorescence intensity of Dr-Vsp (green) and F-actin (red) along the apicobasal axis of individual LREs. Data were presented as mean ± SEM. Enterocytes = 16 cells from 4 larvae for each zebrafish line. The dotted line in the graph conceptually indicates the junction between the microvilli and the cell body.