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Fig. 8
Figure 8. Retinal and optic nerve gliosis in old (two years) myocilin transgenic zebrafish. A rabbit anti-GFAP primary antibody was used to detected Müller glial cells. (A) Wild-type retina and (B) optic nerve. (C,D) Representative transgenic head tissue sections indicating the regions analysed by immunohistochemistry. (E–H) Transgenic retina. (I–L) Transgenic optic nerve. (M) Quantification of GFAP-positive cells in the ganglion cell layer and optic nerve. Four microscopic fields per eye were analyzed (n = four eyes). ***: p < 0.001, Student’s t-test. Arrowheads: GFAP labelling of Müller cells. Scale bars: 50 μm. Autofl.: tissue autofluorescence used for image contrast and anatomical reference; GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer; OA: ocular alterations; OPL: outer plexiform layer; ONL; outer nuclear layer; PHL: photoreceptor layer; R: retina. Tg/+: transgenic; +/+: wild type. The images are representative of the results observed in two zebrafish of each type.
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