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Fig 6
(A) Western blot analysis of Maid (HHM) protein in various transgenic medaka lines overexpressing HHM-GFP. C, control non-transfected medaka. GFP, medaka transfected with control GFP plasmid. Results are representative of three independent experiments. (B) Immunohistochemical staining to detect BrdU in livers of the indicated lines of HHM transgenic medaka treated for 3 weeks with DEN and assayed at 6 months after DEN treatment. (C) Quantitation of the BrdU labeling results for the livers in (B). Arrows indicate BrdU positive cells. Results are the mean ± SD (n = 10 fish/group). *, p<0.05.
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