FIG 6

FIG 6

Characterization of inflammatory response in germ-free zebrafish larvae expose to ETEC. (A) mRNA expression of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β). (B) mRNA expression of genes involved in mitogen-activated protein kinases (MAPK), nuclear transcription factor-kappa B (NF-κB) and Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway, n = 6. GF, WT and ΔluxS, zebrafish larvae in germ-free were exposed to none or to wild-type or ΔluxS LGG at a concentration of 10⁸ CFU/mL for 24 h on 5 dpf, respectively. ETEC, WT+ETEC and ΔluxS+ETEC, zebrafish larvae in germ-free were exposed to none or to wild-type or ΔluxS LGG at a concentration of 10⁸ CFU/mL for 24 h on 5 dpf, respectively, before being exposed to ETEC at a concentration of 10⁸ CFU/mL for 24 h on 6 dpf. P < 0.05 (*), P < 0.01 (**) and P < 0.001 (***).