Fig. 7

Fig. 7 Fig. 7. Inhibition of lesion incision/excision in UV-irradiated embryos preexposed to Al. Embryos preexposed to Al at 15 mg/L for 35 h and nonexposed embryos were separately irradiated with UV (1200 J/m2) to induce NER. The levels of excised oligonucleotides were detected by TdT-mediated end labeling assay. Arrows indicate the positions of 26- and 32-mer size markers. (A) A representative gel image showing the levels of excised oligomers in embryos under each experimental condition. (B) Quantitative analysis of the effects of Al on lesion incision/excision. Each bar graph represents the mean and standard deviation of three separate experiments. The levels of short oligonucleotides in UV-irradiated embryos preexposed to Al were significantly (p < 0.01) lower than those in UV-irradiated embryos nonexposed to Al.