Fig. 6

Fig. 6 Fig. 6. Exposure time-dependent effects of Al on DNA damage recognition activities in zebrafish embryos. Embryos at 1 hpf were exposed to Al at 15 mg/L for different time periods and 6–4PP detection activities in Al-treated and untreated embryos at the same developmental stages were examined by EMSA. (A) A representative gel image showing the effects of Al on 6–4PP sensing activities after different time periods of exposure. (B) Quantitative determination of 6–4PP binding activities in embryos exposed to Al for different time periods. (C) Time-course effects of Al on ddb2 and xpc expression analyzed by real-time RT-PCR. Each bar graph represents the mean and standard deviation of 3–4 separate experiments. *, ** and *** indicate p < 0.05, p < 0.01and p < 0.005, respectively. CE stands for crude extracts.