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Fig. 5
KDM4C depletion worsened acute kidney injury. Study animals receiving kidney ischemia-reperfusion injury were used for this study. Kidney tissues and serum after ischemia-reperfusion injury for the durations indicated in the figure were harvested (n = 8 for each group). (A). Serum BUN and creatinine levels of wild-type and Kdm4c−/− mice after kidney ischemia-reperfusion injury. (B). Masson trichrome staining results of kidney tissues of wild-type and Kdm4c−/− mice after ischemia-reperfusion injury. (C). Representative results of kidney inflammatory cytokines of the study animals, 1 week after ischemia-reperfusion injury. (D). Results of Western blotting for KDM4C in the kidneys of wild-type and Kdm4c−/− mice at the age of 10 weeks. Abbreviation: BUN, blood urea nitrogen; CCL12, C-C motif ligand 12; CXCL1, C-X-C motif ligand 1; FGF, fibroblast growth factor; IGFBP-2, insulin-like growth factor binding protein-2; IL-1R, interleukin-1 receptor; KDM4C, lysine demethylase 4C. KIM-1, kidney injury molecule-1; NGAL, neutrophil gelatinase-associated lipocalin; WT, wide-type. * p < 0.05.