ZFIN Image: Pan et al., 2022, Fig. 3

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Fig. 3

KDM4C depletion impaired autophagy during serum starvation in vitro. HEK293 cells transfected with KDM4C-sh plasmid were used for the study. Cells were harvested after serum starvation at the time points indicated in the figure. (A). Results of Western blotting for autophagy markers, LC3B, BCL1, and BCL2. (B). Results of immunofluorescence staining for LC3B in control and KDM4C knockdown HEK293 cells with or without serum starvation. (C). Relative ratio of fluorescence intensities of GFP-LC3B puncta with WT as the denominator. (D). Results of Western blotting for KDM4C in control and KDM4C knockdown cells. Abbreviation: K/D, knock down; KDM4C, Lysine demethylase 4C; WT, wild-type.

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