Fig. 2
A high throughput multidimensional behavioral test battery (A) workflow depicting protocol of the multidimensional behavioral test battery. Zebrafish were exposed to toxicants in the embryonic stage (5 hpf) through to free swimming larval stage (5 dpf), with media exchanges occurring daily. The larval zebrafish then underwent the automated battery comprised of the spontaneous swimming (SS), simulated predator response (SPR), larval photomotor response (LPR) assays as well as a new thermotaxis (TX) biotests; (B) Overview of the purpose build analytical system used in the multidimensional behavioral test battery; (C) A photic module used for SPR and LPR stimuli biotests, (D) An electrothermal module used for thermotactic biotests.