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Fig. 5
Apoptotic effects caused by 3d. HeLa cells were treated with the indicated concentrations of 3d for either 24 or 48 h and then were analyzed by flow cytometry after double staining with annexin-V-FITC and PI. A−/PI− Alive cells; A+/PI− Early apoptotic cells; A+/PI+ Late apoptotic cells; A−/PI+ Necrotic cells. In the histograms, data are represented as mean ± SEM of three independent experiments.
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