IMAGE

Fig. 5

ID
ZDB-IMAGE-220824-70
Source
Figures for Lavorato et al., 2022
Image
Figure Caption

Fig. 5 Biochemical analysis, histopathology, and ultrastructural investigation of homozygous fbxl4sa12470 zebrafish liver. (A) Liver histology in AB (WT) zebrafish larvae at 6 dpf. Scale bar: 25 μm. (B) Vacuolated liver in homozygous fbxl4sa12470 zebrafish larvae at 6 dpf. 100% of larvae (n = 25) showed vacuolated liver, while no WT larvae (n = 12) showed the disease phenotype. Scale bar: 25 μm. (C) Ultrastructure of hepatocytes in 7 WT dpf zebrafish larvae showing normal ultrastructure. Scale bar: 10 μm. (D) Ultrastructure of hepatocytes in mutant 7 dpf zebrafish larvae showing increased rate of lipid droplets (L) and autophagic vacuoles (white arrows). Scale bar: 10 μm. (E) Total area of vacuoles occupying liver areas estimated in μm2 in mutant versus WT larvae at 7 dpf. Total area of liver analyzed: 1,978 μm2 (WT) and 2,741 μm2 (fbxl4sa12470). **P < 0.01. Significance was determined using unpaired t test. n = 3 each fish line. (F) Mitochondrial ultrastructure (M) in hepatocytes of 7 dpf WT zebrafish larvae. (G) Loss of normal matrix electron density and mitochondrial cristae damage (M) was observed in hepatocytes of 7 dpf fbxl4sa12470–/– larvae. Scale bar: 1 μm (F and G).

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ JCI Insight