Fig. 1

Fig. 1

LncRNA STEAP3-AS1 is transcriptionally induced by HIF-1? under hypoxia. A Schematic diagram describing the screening process of candidate antisense lncRNAs using the TCGA dataset. B The correlation between STEAP3-AS1 and HIF1A RNA level in TCGA datasets was analyzed by Pearson correlation test. C The expression of STEAP3-AS1 in normal and CRC samples from the TCGA datasets. D Kaplan?Meier analysis of progression free survival of CRC patients with low or high STEAP3-AS1 expression according to the TCGA dataset (P = 0.037, log-rank test). E qPCR was performed to determine relative STEAP3-AS1 RNA level in DLD-1 and SW480 cells after treatment with 1% O₂ for 0 h, 4 h and 8 h. F-G Relative STEAP3-AS1 expression in DLD-1 and SW480 cells treated with DMOG (1 mM) or CoCl₂ (100 ?M) for 48 h was determined by qPCR. H 200 SW480 cells expressing mCherry were implanted into the perivitelline space of 3dpf flk:eGFP Casper zebrafishes. After being under normoxic or hypoxic (8% O₂) condition for 3 days, the zebrafishes were then monitored by stereo microscopy. Scale bar: 250 ?m. I qPCR was performed to determine the relative RNA levels of STEAP3-AS1, HIF1A, VEGFA, PGK1, SLC2A3 in mCherry SW480-derived zebrafish xenograft models with or without hypoxic treatment. J ChIP assay investigating the binding capacity of HIF-1? to each HRE was conducted in DLD-1 and SW480 cells. K FISH assay was conducted to determine the subcellular location of lncRNA STEAP3-AS1 (Cy3) in DLD-1 and SW480 cells. DAPI-stained nuclei are blue. Scale bar: 10 ?m. L The expression level of lncRNA STEAP3-AS1 in the subcellular fractions of DLD-1 cells was detected by qPCR. U6 and GAPDH were used as nuclear and cytoplasmic markers, respectively. Data are means ± s.d. and are representative of at least 3 independent experiments. (* P < 0.05, ** P < 0.01, and *** P < 0.001)