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Fig. 6
Cfap53 facilitates assembly of the zygotic spindle. (A,B) Maximal projections of confocal stacks on Tg(XlEef1a1:dclk2a-GFP)/MPcfap53?/? embryos at 30 mpf and immunolabeled for GFP, ?-tubulin and DAPI. While six embryos formed centrosomes and a normal mitotic spindle during the first zygotic cell division (A, n=6), six embryos failed to assemble the centrosomes and the mitotic spindle (B, n=6). Instead, microtubule originating from the chromosomes is observed in these embryos. Scale bars: 20??m.
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