Figure 6

Figure 6

Phenotypic analysis of the L1CAM^Q497H knockin mice. (A) Flow cytometric analysis of the frequencies of thymocytes from wildtype (WT) and hemizygous L1cam^497H mutant mice. (B) The total and subpopulations of thymic cellularity are shown in bar graphs. The following populations are graphed: DN, CD4^-CD8^-; DP, CD4⁺CD8⁺; CD4⁺; CD8⁺; DN1, CD4^-CD8^-CD25^-CD44⁺; DN2, CD4^-CD8^-CD25⁺CD44⁺; DN3, CD4^-CD8^-CD44^-CD25⁺; DN4, CD4^-CD8^-CD44^-CD25^-. (C) Flow cytometry analysis of splenic CD4⁺ and CD8⁺ T cells, B (B220⁺IgM⁺) and NK (CD3^-CD122⁺NK1.1⁺) cells in WT and L1cam^Q497H mutant mice. (D) The total and subpopulations of splenic cellularity are shown in bar graphs. (E) Flow cytometry analysis of CD44 and CD62L staining of CD4⁺ and CD8⁺ T cells. (F) The frequency of CD44⁺CD62L⁺ in CD4⁺ and CD8⁺ T cells is shown in bar graphs. Data are representative of 2 independent experiments performed. No statistically significant differences were found in any of the indicated populations.