Fig. 3

Fig. 3

Fig. 3. A determinant in ISG15 contributes to specific ISG15-Uba7 signaling. (A) Alignment of ISG15 sequences from selected species. The multiple alignments were produced using ClustalW. The positions that are important for the properties of ISG15 are highlighted with blue boxes. (B) zUba1 activates Myc-Ub and Myc-Ub^{R123W, I125T}. The reactions were prepared at the indicated time points, and immunoblotting was done with anti-Myc. (C, D) zUba1 activates myc-zISG15 and its double-point (C) or single-point (D) mutants; the reactions were prepared at the indicated time points. (E) zUba1 can activate hISG15 ^T125I, hISG15^W123R, and hISG15 ^{I125T, W123R} more than wild-type hISG15^WT. (F, G) zUba1 activates aISG15 ^L125I, aISG15^W123R (F), and aISG15 ^K1K150V (G) more than wild-type aISG15^WT. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)