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Fig. 3

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ZDB-IMAGE-220811-3
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Figures for Yan et al., 2022
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Fig. 3

Fig. 3. In the absence of Irf2bp2a, p53 pathway is aberrantly activated due to impaired turnover, which contributes to the defective liver development. (A) WISH assay of lfabp at 72 hpf in irf2bp2a−/− embryos, and irf2bp2a−/− embryos injected with p53 MO. (C) WISH assay of lfabp in WT, WT embryos injected with irf2bp2a MO, p53−/−, and p53−/− embryos injected with irf2bp2a MO. (B, D) The relative liver area measured. The data shown is fold difference compared with the level detected in control embryos. p values are denoted by asterisks. (Student t-test, N ≥ 3. Error bars represent mean ± SD, ns, not significant, ****P < 0.0001). (E) IRF2BP2 and P53 mRNA levels were measured by RT-qPCR in HepG2 cells after transfection of an irrelevant shRNA (NC, non-specific control) or a shRNA against IRF2BP2 (sh-IRF2BP2). (Student t-test, N ≥ 3. Error bars represent mean ± SD, ns, not significant, ***P < 0.001). (F) Western blot analysis of IRF2BP2, P53, IRF2 and MDM2 in WT and IRF2BP2-knockdown HepG2 cells. (G) IRF2 protein was immunoprecipitated (IP) with an anti-IRF2 antibody in WT and IRF2BP2-knockdown HepG2 cells, then MDM2 protein was detected by western blot analysis. (H) WISH assay of lfabp in WT, WT embryos injected with full length irf2 or irf2bp2a mRNA.

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Reprinted from Biochimica et biophysica acta. General subjects, 1866(10), Yan, L., Gao, S., Zhu, J., Zhou, J., Irf2bp2a regulates liver development via stabilizing P53 protein in zebrafish, 130186, Copyright (2022) with permission from Elsevier. Full text @ BBA General Subjects