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Fig. 2

ID: ZDB-IMAGE-220804-16
Publication: Delnoy et al., 2022 - Novel mRNA therapy restores GALT protein and enzyme activity in a zebrafish model of classical galactosemia
All Figures: Figures for Delnoy et al., 2022

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Fig. 2

hGALT expression and activity in 5 dpf CG zebrafish (galt KO) after single dose hGALT mRNA injection (100 ng/μl) at the one-cell stage. A: Western blot analysis: Non-injected WT and CG zebrafish (galt KO) controls present no band, which indicates that the antibody is specific for hGALT. Samples were loaded in duplicate (n = 150 per sample). B: GALT activity (nmol/mg protein/h), results are presented as mean ± SEM. All samples (n = 3 per experimental group, on average 30 zebrafish/sample), were measured in duplicate. MW, molecular weight ladder; WT, wildtype; NIC, non-injected CG zebrafish (galt KO) control; NKD, naked mRNA injected CG zebrafish (galt KO); LNP1, LNP1-packaged hGALT mRNA injected CG zebrafish (galt KO); LNP2, LNP2-packaged hGALT mRNA injected CG zebrafish (galt KO)

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Inherit. Metab. Dis.