ZFIN Image: Russo et al., 2022, Fig. 8

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Figure Caption

Fig. 8

Antibody stability assessment.

(A) Size exclusion chromatography (SEC) analysis of different Hyper-Myc IgG variants with constant parts of human IgG1 (left panel), rabbit IgG (middle panel) or mouse IgG2a (right panel). (B) Shelf-life of Myc1-9E10 hybridoma antibody was compared to the three different versions of Hyper-Myc. Each antibody was stored for a period of seven months at 4 °C prior testing in triplicate in ELISA. An aliquot stored at −80 °C for the same period of time was freshly thawed and used as reference. The aliquots did not contain any protectants nor stabilizers. Error bars represent the standard deviation. Asymmetric Sigmoidal, five parameter logistic (5 PL) curve fitting was used to determine the EC₅₀ values.

Acknowledgments

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