ZFIN Image: Russo et al., 2022, Fig. 4

Image

Figure Caption

Fig. 4

Flow cytometry analysis of myc-tagged antibodies bound to cells.

(A) Histograms of Daudi cells stained with either Myc1-9E10 (top panel) or Hyper-Myc (bottom panel) in the presence or absence of primary anti-CD19 scFv myc-tagged antibody fragment. Histograms are shown for concentrations of 2 μg/mL of Myc1-9E10 or Hyper-Myc, or 6.32 μg/mL for the negative controls. (B) Sensitivity of detection of myc-tagged anti-CD19 scFv on Daudi cells followed by incubation with either Hyper-Myc (red) or Myc1-9E10 (black) in concentrations ranging from 6.32 ng/mL to 6.32μg/mL. Alive single cells were gated; APC-conjugated goat anti-mouse IgG Fc was used for visualisation, and the median of the APC signal was measured using a MACSQuant flow cytometer. Error bars represent the standard deviation of duplicates.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biol. Chem.