ZFIN Image: Shang et al., 2022, Figure 6.

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Figure Caption

Figure 6.

Structural remodeling of the apical stem can fully rescue defective mir-144 variants. (A) Schematics of 2bp insertion variants of mir-144 mutants. The wildtype mir-144 loop exhibits a 19 bp stem to the Dicer cleavage site on the 3p arm (Figure 2A). As shown in Figure 2, insertion of a 2bp stem just distal to the Dicer cleavage site, rendering a 21 bp stem, impairs production of pre-mir-144, but results in a relative enhancement of mature miR-144-3p yield. The green bar indicates similar 2 bp stem insertions into other mir-144 mutants that are incompetent for Dicer cleavage. (B) Northern blotting of the panel of unmodified mir-144 mutants (labeled red) and their companion 2 bp insertion variants shows rescue of mature miR-144 production. (C) Luciferase sensor assays shows rescue of miR-144-3p activity by 2bp insertions in all defective mir-144 variants.

Acknowledgments

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