ZFIN Image: Ciampi et al., 2022, Fig. 2

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Figure Caption

Fig. 2

Evolutionary study of RetMICs. (A) Number of tissue-enriched microexons (as in Fig. 1A) in mouse, chicken, and zebrafish. Only the four tissues with the highest number of tissue-enriched microexons are represented. (B) Genomic conservation of human RetMICs (full color, left bar) and human RetLONGs (transparent color, right bar) in mouse, chicken, and zebrafish. Human RetMICs/RetLONGs are considered genomically conserved in another species when they belong to an exon orthogroup including at least one exon from that species. Asterisks indicate significant differences between RetMICs and RetLONGs conservation (P < 0.05, hypergeometric test). (C) Heatmap showing the bias in retina inclusion for genomically conserved human RetMICs (top row) and their respective orthologs in mouse, chicken, and zebrafish. Each column corresponds to a different RetMIC, and the color represents the ΔPSI between the average of the retina samples and of all the other tissues, with darker blue reflecting greater retina inclusion bias. In case of multiple orthologs, only the one with the highest ΔPSI (retina − others) was plotted. Blanks and ivory rectangles indicate missing orthologs and missing ΔPSI values due to lack of read coverage, respectively. (D) Dot plot representing functional enrichment of RetMIC-containing genes across species. The functional enrichment of genes containing RetMICs was separately tested for each of the species, and significant categories in at least two species (FDR-adjusted P ≤ 0.05) were plotted. The color reflects the adjusted P value, with yellow color depicting P ≥ 0.05. The size of the dots is proportional to the log₂ of the observed vs. expected ratio (O/E), and black borders around the categories highlight log₂ O/E ≥ 1. PMD, plasma membrane bounded.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA