ZFIN Image: Zasu et al., 2022, Fig. 4

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Fig. 4

Nox-mediated generation of H₂O₂ is crucial for the hypoxia/re-oxygenation-induced Erk1/2-phosphorylation. (A) Time-course Erk1/2 phosphorylation changes of the VAS2870-treated embryos. Outline of experimental schedule is shown above the data. Reoxy embryos treated with or without VAS2870 were sampled at various timing, and the Erk1/2-phosphorylation levels were tested by immunoblotting. Relative Erk1/2-phosphorylation levels were shown as mean ± SE of 2-3 independent assays. (B) Erk1/2- and Akt- phosphorylation changes of the VAS2870-treated embryos. Embryos treated with or without VAS2870 (500 nM) were sampled at 48 hpf (16 hr post-re-oxygenation), and the Erk1/2- and Akt-phosphorylation levels were tested by immunoblotting. Relative Erk1/2- and Akt- phosphorylation levels were shown as mean ± SE of 2-3 independent assays. The asterisk (*) denotes statistical difference at P<0.05.

Acknowledgments

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